ABSTRACT
The chemical constituents from the roots of Aconitum kongboense were studied. Twenty-five diterpenoid alkaloids were isolated from the 95% methanol extract of the roots of A. kongboense by silica gel, reverse-phase silica gel and basic alumina column chromatography. They included a new aconitine-type diterpenoid alkaloid, named as kongboensenine(1), and twenty-four known ones(2-25), i.e., acotarine F(2), acotarine G(3), 14-acetyltalatisamine(4), talatisamine(5), indaconitine(6), yunaconitine(7), chasmanine(8), 6-epi-foresticine(9), homochasmanine(10), 8-deacetyl-yunaconitine(11), chasmaconitine(12), ajaconine(13), franchetine(14), ezochasmanine(15), crassicautine(16), 14-O-deacylcrassicausine(17), genicunine A(18), falconeridine(19), sachaconitine(20), liljestrandisine(21), 8-methyl-14-acetyltalatisamine(22), kongboendine(23), 14-benzoylchasmanine(24) and pseudaconine(25). Their structures were elucidated by common spectroscopic methods including high-resolution electrospray ionization mass spectrometry(HR-ESI-MS) and nuclear magnetic resonance(NMR) techniques. Compounds 2-4, 10, 13, 15-19 and 21-22 were isolated from this plant for the first time. Experimental results showed that all compounds did not have a significant inhibitory activity against acetylcholinesterase(AChE).
Subject(s)
Acetylcholinesterase , Aconitum/metabolism , Alkaloids , Diterpenes , Magnetic Resonance Spectroscopy , Molecular Structure , Plant Roots/metabolismABSTRACT
In the present study, three new aconitine-type diterpenoid alkaloids brochyponines A-C (1-3) were isolated from the roots of Aconitum brevicalcaratum. Their structures were established on the basis of extensive spectroscopic analyses (IR, HR-ESI-MS, and 1D and 2D NMR). The NMR data of salt form for compound 1 in CDCl were also measured.
Subject(s)
Aconitum , Chemistry , Alkaloids , Chemistry , Drugs, Chinese Herbal , Chemistry , Magnetic Resonance Spectroscopy , Molecular Structure , Plant Roots , ChemistryABSTRACT
In the present study, three new aconitine-type diterpenoid alkaloids brochyponines A-C (1-3) were isolated from the roots of Aconitum brevicalcaratum. Their structures were established on the basis of extensive spectroscopic analyses (IR, HR-ESI-MS, and 1D and 2D NMR). The NMR data of salt form for compound 1 in CDCl were also measured.
Subject(s)
Aconitum , Chemistry , Alkaloids , Chemistry , Drugs, Chinese Herbal , Chemistry , Magnetic Resonance Spectroscopy , Molecular Structure , Plant Roots , ChemistryABSTRACT
Eight new C-diterpenoid alkaloid arabinosides, named aconicarmichosides E-L (-), were isolated from an aqueous extract of the lateral roots of (Fu Zi). Their structures were determined by spectroscopic and chemical methods including 2D NMR experiments and acid hydrolysis. Compounds -, together with the previously reported four neoline 14--arabinosides from the same plant, represent the only examples of glycosidic diterpenoid alkaloids so far. At a dose of 1.0 mg/kg (i.p.), as compared with the black control, compounds , , and - exhibited analgesic effects with >65.6% inhibitions against acetic acid-induced writhing of mice. Structure-activity relationship was also discussed.
ABSTRACT
Objective To investigate the effects of human IL-18 gene combined with diterpenoid alkaloids in inhibiting the proliferation and inducing the apoptosis of tongue squamous carcinoma cells Tscca.Methods We constructed recombinant plasmid pEGFPN3-IL-18 and tranfected it into tongue squamous carcinoma cells Tscca.The transduction efficiency of the target cells was detected by fluorescent microscopy,cytotoxic effect of IL-18 gene with diterpenoid alkaloids on Tscca was detected by MTT assay,and apoptosis was detected by flow cytometry. Western blot was employed to examine the expression level of cellular signal-regulated kinase Akt/p-Akt.Results The tongue squamous cells Tscca which transfected pEGFPN3-IL-18 had significantly increased apoptosis compared with non-transfected cells (P<0 .05 ).Tongue carcinoma squamous cells cultured with diterpenoid alkaloids at the concentrations of 0 .2 ,0 .4 and 0 .6 mg/mL had significantly increased apoptosis in a dose-dependent manner (P<0.05).Human IL-18 gene combined with diterpenoid alkaloids for 48 hours inhibited significantly Tscca in a concentration-dependent manner compared with diterpenoid alkaloids alone (P<0 .05 ).The two in combination could also decrease the protein level of p-Akt dose-dependently.Conclusion The combination of pEGFPN3-IL-18 and diterpenoid alkaloids has a synergistic effect in inhibiting the growth of tongue squamous carcinoma cells Tscca.
ABSTRACT
Objective: To investigate the diterpenoid alkaloids from Aconitum episcopale. Methods: The obtained compounds were isolated by different chromatographic methods and their structures were identified by physicochemical properties and spectral data. Results: Ten diterpenoid alkaloids were isolated and identified as talatizamine (1), chasmanine (2), crassicauline A (3), foresaconitine (4), acoforestinine (5), yunaconitine (6), 3-deoxy-8-deacetyl-yunaeonitin (7), leucanthumsine D (8), pengshenine B (9), and macrorhynine B (10). Conclusion: Compounds 2-5 and 7-10 are isolated from this plant for the first time.
ABSTRACT
Object To search for more bioactive alkaloids from Aconitum coreanurn through this fur-ther study. Methods The ethanolic extract from the root of A. coreanum was isolated by aluminium oxidecolumn chromatography, the constituent structures were elucidated mainly by their physicochemical prop-erties and spectroscopic (IR, ESI-MS,1 H-NMR, 13 C-NMR,1 H-1 HCOSY, HMQC, and HMBC)analysis.Results Seven alkaloids were obtained from the root of A. coreanum, they were identified as: 11, 13-di-acetyl-14-hydroxy-2-isobutyryl hetisine ( Ⅰ ), guan-fu base G ( Ⅱ ), guan-fu base F ( Ⅲ ), guan-fu base Z( Ⅳ ), guan-fu base I ( Ⅴ ), guan-fu base K ( Ⅵ ), and guan-fu aminealcohol (Ⅶ). Conclusion Compound I is a new natural product, named as guan-fu base P, its 1 H-NMR and 13 C-NMR data are reported first inthis paper.